This application note describes the extraction of cocaine and major metabolites from whole blood, prior to GC/MS analysis. This protocol also allows the simultaneous extraction of various other drugs of abuse classes: amphetamines, barbiturates, benzodiazepines and opiates.
ISOLUTE® SLE+ columns with 1 mL sample capacity are used to extract whole blood samples following a straightforward sample dilution. No protein precipitation or other pre-treatment is required prior to sample loading. The sample preparation procedure delivers clean extracts, good recoveries and RSD values and LLOQs from 20 ng/mL (analyte dependant).
Tags: Anhydroecgonine Methyl Ester AEME , Anhydroecgonine methyl ester , Application Notes , Benzoylecgonine , Clinical , Cocaethylene , Cocaine , Cocaines , Column , Drugs of Abuse , Ecgonine Methyl Ester EME , English , Forensic , GC-MS , Whole blood