Cardiovascular effects of PACAP and PACAP(6â38) in the RVLM of normotensive and hypertensive rats. A and B: changes in mean arterial pressure (MAP; i), HR (ii), and percentage of sSNA (iii) before and after the administration of PACAP (A) or PACAP(6â38) (B). Arrows indicate times of drug infusion. âPBSâ indicates the period after the bilateral RVLM microinjection of PBS; âPACAPâ and âPACAP(6â38)â indicate the periods after the bilateral RVLM microinjection of PACAP or PACAP(6â38), respectively. C: comparison of maximum MAP (i), HR (ii), and percentage of sSNA responses (iii) after PACAP or PACAP(6â38). *P < ; **P < ; ***P < .
Trifluoroacetic acid (TFA) is a strong acid commonly used to cleave synthesized peptides from solid-phase resins and improve HPLC performance during peptide purification. Most custom peptides are delivered as lyophilized TFA salts, containing as much as 10-45% TFA. Residual TFA in peptide samples can cause cytotoxicity in cell culture assays, therefore TFA removal service is recommended for peptides that will be used in cellular assays. We provide both standard and guaranteed (TFA < 1%) TFA removal service to meet your specific research needs.
More about TFA Removal for Peptides
In addition to peptide synthesizers, CEM also offers a unique system for cleavage of peptides from resin. This system, Razor, provides for parallel cleavage of up to 12 peptides at elevated temperature with an integrated manifold for draining into collection vials. Using the Razor cleavage times can be reduced from 3-4 hours down to only 30 minutes with more complete removal of difficult side chain protecting groups such as Pbf and tBu. In addition to instrumentation, CEM offers a complete line of reagents including Fmoc amino acids, pre-loaded resins, and other reagents.